珍稀濒危物种堇叶紫金牛高效快繁体系的建立

筛选堇叶紫金牛(Ardisia violacea)野生优株,以其当年新发带休眠腋芽茎段为外植体,通过启动培养、丛生芽诱导增殖、壮苗培养、生根培养和炼苗移栽等过程建立其组培快繁技术体系。研究结果表明,最佳启动培养基为MS+0.80 mg·L~(–1)KT+0.10 mg·L~(–1) NAA+0.10 mg·L~(–1) IBA,腋芽萌发率达92.60%;最佳丛生芽诱导增殖培养基为MS1+0.50 mg·L~(–1) TDZ+0.10mg·L~(–1) NAA,平均增殖系数达8.60;最佳壮苗培养基为MS+1.00 mg·L~(–1) KT+0.50 mg·L~(–1) NAA;最佳生根培养基为1/2MS+2.00 mg·L~(–1) IBA+1.00 mg·L~(–1) NAA+1.00 mg·L~(–1) AC,平均生根率达98.70%;采用松鳞和泥炭(2:1,v/v)作为炼苗基质,炼苗成活率可达85.30%。实验成功建立了堇叶紫金牛高效组培快繁技术体系,经验证该体系能够满足规模化生产的需求。

Establishment of a Tissue Culture and Propagation System for Ardisia violacea,a Rare and Endangered Species

Shoot segments with axillary buds from young branches of wild Ardisia violacea with excellent characters were cultured in vitro. Through bud induction, multiple shoots proliferation, subculture, rooting and transplantation, a regeneration system was established. The optimal bud induction medium was MS+0.80 mg?L^-1 KT+0.10 mg?L^-1 NAA+0.10 mg?L^-1 IBA, and the germination frequency of axillary buds reached 92.60%; the optimal multiple shoot proliferation medium was MS1+0.50 mg?L^-1 TDZ+0.10 mg?L^-1 NAA, and the average proliferation coefficient reached 8.60; the optimal subculture medium was MS+1.00 mg?L^-1 KT+0.50 mg?L^-1 NAA; the optimal rooting medium was 1/2MS+2.00 mg?L^-1 IBA+1.00 mg?L^-1 NAA+1.00 mg?L^-1 AC, and the average frequency of shoot rooting reached 98.70%. Using pine bark:peat (2:1, v/v) as a matrix, the transplant survival rate could reach 85.30%. We established a high-frequency regeneration system for A. violacea that could meet the needs of large-scale production.