| 以黄花乌头发根为外植体的再生培养体系建立 |
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| 引用本文： | 刘莉莉,卢淑波,徐佳萍,张庆田,李昌禹.以黄花乌头发根为外植体的再生培养体系建立[J].植物学报,2015,50(5):623-627. |
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| 作者姓名： | 刘莉莉 卢淑波 徐佳萍 张庆田 李昌禹 |
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| 作者单位： | 中国农业科学院特产研究所, 长春 130112 |
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| 基金项目： | 吉林省科学技术厅自然科学基金(No.201115131) |
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| 摘 要： | 为获得黄花乌头(Aconitum coreanum)发根再生植株, 将其发根在添加0.5-5.0 mg·L-1 6-BA及0-0.5 mg·L-1 NAA的1/2MS固体培养基上进行光诱导培养, 发根经脱分化形成愈伤组织, 然后形成芽及芽丛, 最终获得发根再生植株。结果表明, 愈伤组织及不定芽分化的最适培养基为1/2MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA+3%蔗糖; 不定芽生根最适培养基为1/2MS+0.5 mg·L-1 6-BA+0.1 mg·L-1 NAA+5 mg·L-1 GA+3%蔗糖。
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| 收稿时间： | 2014-09-22 |
Establishment of Plant Regeneration System from Hairy Roots of Aconitum coreanum |
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| Lili Liu,Shubo Lu,Jiaping Xu,Qingtian Zhang,Changyu Li.Establishment of Plant Regeneration System from Hairy Roots of Aconitum coreanum[J].Bulletin of Botany,2015,50(5):623-627. |
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| Authors: | Lili Liu Shubo Lu Jiaping Xu Qingtian Zhang Changyu Li |
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| Institution: | Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China |
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| Abstract: | The hairy roots of Aconitum coreanum were used as explants to induce callus via differentiation in 1/2MS medium with 0.5-5 mg·L-1 6-BA and 0-0.5 mg·L-1 NAA, then regeneration plantlets were induced from the callus. A whole transgenetic plantlet was obtained. The optimal medium for inducing callus and adventitious buds was 1/2MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA+3% sugar. The optimal medium for inducing roots was 1/2MS+0.5 mg·L-1 6-BA+0.1 mg·L-1 NAA+5 mg·L-1 GA+3% sugar. |
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