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| 拟南芥IAA酰胺合成酶GH3-6负调控干旱和盐胁迫的反应 | |
| 引用本文： | 刘晓东,王若仲,焦彬彬,代培红,李月.拟南芥IAA酰胺合成酶GH3-6负调控干旱和盐胁迫的反应[J].植物学报,2016,51(5):586-593. |
| 作者姓名： | 刘晓东 王若仲 焦彬彬 代培红 李月 |
| 作者单位： | 新疆农业大学农学院, 乌鲁木齐 830052 湖南农业大学植物激素与生长发育湖南省重点实验室, 长沙 410128 上海出入境检验检疫局, 上海 200135 |
| 基金项目： | 国家自然科学基金(No.31470289) |
| 摘 要： | 生长素是一种重要的植物激素,几乎参与了植物所有的生命活动过程。GH3-6具有IAA酰胺合成酶活性,催化氨基酸与IAA形成IAA的氨基轭合物,发挥暂时或永久灭活IAA的作用。该文探讨了GH3-6基因在拟南芥(Arabidopsis thaliana)逆境适应过程中的功能。结果显示GH3-6基因受干旱、ABA和高盐的诱导表达。与野生型相比,GH3-6基因过表达突变体dfl1-D对干旱的抗性明显减弱,叶片失水速率更快。在抗盐方面,dfl1-D也显著弱于野生型。在3种逆境(干旱、ABA和高盐)胁迫下,GH3-6基因的高表达抑制了逆境响应基因RD22、KIN1、RD29A和DREB1A的表达。而且在干旱胁迫下,dfl1-D中ABA的含量明显低于野生型。研究结果证明,高表达GH3-6基因负调控拟南芥对逆境的抗性。 |
| 收稿时间： | 2015-12-22 |
Indole Acetic Acid-Amido Synthetase GH3-6 Negatively Regulates Response to Drought and Salt in Arabidopsis |
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| Xiaodong Liu,Ruozhong Wang,Binbin Jiao,Peihong Dai,Yue Li.Indole Acetic Acid-Amido Synthetase GH3-6 Negatively Regulates Response to Drought and Salt in Arabidopsis[J].Bulletin of Botany,2016,51(5):586-593. | |
| Authors: | Xiaodong Liu Ruozhong Wang Binbin Jiao Peihong Dai Yue Li |
| Institution: | College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China |
| Abstract: | Auxin is an important phytohormone involved in almost all aspects of plant life. GH3-6 encodes a protein possessing adenylation activity that can conjugate amino acid to indole acetic acid (IAA), which leads to temporarily or permanently inactive IAA. Here we investigated the role of GH3-6 in plant stress adaptation. The expression of GH3-6 was induced by drought, abscisic acid (ABA) and high salt. Compared with the wild type, transgenic plants with GH3-6 overexpression (named dfl1-D) were sensitive to drought and lost more water from detached rosette leaves. dfl1-D also exhibited hyporesistance to high salt. In addition, overexpression of GH3-6 inhibited the expression of stress-responsive genes, including RD22, KIN1, RD29A and DREB1A. The content of ABA was lower in dfl1-D than the wild type under drought treatment. Overexpression of GH3-6 negatively regulates stress resistance in Arabidopsis. |
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