The binding of decomposition products of UDP-galactose to the microsomes and polyribosomes isolated from rat liver |
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Authors: | T Kopacz-Jodczyk W Ga?asiński |
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Institution: | Department of General and Organic Chemistry, Institute of Chemistry, Medical School, Bia?ystok, Poland. |
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Abstract: | UDP-D-U-14C]galactose is decomposed to U-14C]galactose-1-phosphate and U-14C]galactose by rat liver microsomal and crude polyribosomal fractions, under conditions commonly used to assay of glycosyltransferase activities. UDP-D-U-14C]galactose, at neutral pH, is also chemically degraded to the U-14C]galactose-1,2-cyclic phosphate. The 1,2-cyclic phosphate derivative of galactose also exists in the commercial UDP-D-U-14C]galactose. It is a very important finding that products of the UDP-D-U-14C]galactose decomposition are tightly, although nonenzymatically, bound to tested subcellular fractions and may create a false impression of protein glycosylation. The application of controls containing all radioactive substances present in suitable samples is recommended in order to avoid incorrect interpretations of the results. |
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