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A compendium of methods useful for characterizing Arabidopsis pollen mutants and gametophytically-expressed genes
Authors:Johnson-Brousseau Sheila A  McCormick Sheila
Institution:Plant Gene Expression Center and Plant and Microbial Biology, USDA/ARS and UC-Berkeley, 800 Buchanan St., Albany, CA 94710, USA. sjb@uclink.berkeley.edu
Abstract:This article provides detailed protocols for collecting pollen and outlines genetic crosses and phenotypic assays that are useful for characterizing mutants that affect pollen development.
Keywords:gametophyte  mutant              quartet                        tetraspore            segregation distortion  pollen germination
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