Evidence for a role of β-1,3-glucanase in dicot seed germination |
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Authors: | Regina Vögeli-Lange Corinne Fründt Craig M Hart Roland Beffa Ferenc Nagy Frederick Meins Jr |
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Institution: | Friedrich Miescher-Institut, Postfach 2543, CH-4002 Basel, Switzerland |
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Abstract: | Class I β-1,3-glucanases are antifungal vacuolar proteins implicated in plant defense that show developmental, hormonal, and pathogenesis-related regulation. The expression was studied in germinating tobacco seeds of a chimeric β-glucuronidase (GUS) reporter gene fused to 1.6 kb of the 5' flanking sequence of the tobacco class I β-1,3-glucanase B (GLB) promoter. Histological staining for GUS activity showed that expression of the GLB promoter is highly localized in a specific zone of the endosperm in germinating seeds. The temporal and spatial patterns of GUS and β-1,3-glucanase activity found, suggest a novel function for class I β-1,3-glucanases during seed germination in a dicotyledonous plant. |
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