RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana |
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| Authors: | Rikard Fristedt Chen Hu Nicole Wheatley Laura M Roy Rebekka M Wachter Linda Savage Jeremy Harbinson David M Kramer Sabeeha S Merchant Todd Yeates Roberta Croce |
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| Institution: | 1. Biophysics of Photosynthesis, VU University Amsterdam, Amsterdam, The Netherlands;2. Department of Chemistry and Biochemistry and Institute for Genomics and Proteomics, UCLA, Los Angeles, CA, USA;3. School of Molecular Sciences and Center for Bioenergy and Photosynthesis, Arizona State University, Tempe, AZ, USA;4. Department of Energy Plant Research Laboratory, Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, USA;5. Horticulture and Product Physiology Group, Wageningen University, Wageningen, The Netherlands |
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| Abstract: | Ribulose‐1,5‐bisphosphate carboxylase/oxygenase (RuBisCO) catalyzes the reaction between gaseous carbon dioxide (CO2) and ribulose‐1,5‐bisphosphate. Although it is one of the most studied enzymes, the assembly mechanisms of the large hexadecameric RuBisCO is still emerging. In bacteria and in the C4 plant Zea mays, a protein with distant homology to p terin‐4α‐c arbinolamine d ehydratase (PCD) has recently been shown to be involved in RuBisCO assembly. However, studies of the homologous PCD‐like protein (RAF2, RuBisCO assembly factor 2) in the C3 plant Arabidopsis thaliana (A. thaliana) have so far focused on its role in hormone and stress signaling. We investigated whether A. thalianaRAF2 is also involved in RuBisCO assembly. We localized RAF2 to the soluble chloroplast stroma and demonstrated that raf2 A. thaliana mutant plants display a severe pale green phenotype with reduced levels of stromal RuBisCO. We concluded that the RAF2 protein is probably involved in RuBisCO assembly in the C3 plant A. thaliana. |
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| Keywords: | abscisic acid Atg5 g51110 chloroplast RAF2 RuBisCO RuBisCO aggregation RuBisCO assembly factor SDIRIP1 |
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