A B-lectin receptor kinase gene conferring rice blast resistance |
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Authors: | Chen Xuewei Shang Junjun Chen Dexi Lei Cailin Zou Yan Zhai Wenxue Liu Guozhen Xu Jichen Ling Zhongzhuan Cao Gang Ma Bingtian Wang Yuping Zhao Xianfeng Li Shigui Zhu Lihuang |
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Institution: | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. |
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Abstract: | Rice blast, caused by the fungal pathogen Magnaporthe grisea, is one of the most devastating diseases in rice worldwide. The dominant resistance gene, Pi-d2 previously named Pi-d(t)2], present in the rice variety Digu, confers gene-for-gene resistance to the Chinese blast strain, ZB15. Pi-d2 was previously mapped close to the centromere of chromosome 6. In this study, the Pi-d2 gene was isolated by a map-based cloning strategy. Pi-d2 encodes a receptor-like kinase protein with a predicted extracellular domain of a bulb-type mannose specific binding lectin (B-lectin) and an intracellular serine-threonine kinase domain. Pi-d2 is a single-copy gene that is constitutively expressed in the rice variety Digu. Transgenic plants carrying the Pi-d2 transgene confer race-specific resistance to the M. grisea strain, ZB15. The Pi-d2 protein is plasma membrane localized. A single amino acid difference at position 441 of Pi-d2 distinguishes resistant and susceptible alleles of rice blast resistance gene Pi-d2. Because of its novel extracellular domain, Pi-d2 represents a new class of plant resistance genes. |
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Keywords: | B-lectin serine–threonine kinase resistance gene map-based cloning rice blast rice (Oryza sativa L ) |
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