Serine/threonine kinase activity in the putative histidine kinase-like ethylene receptor NTHK1 from tobacco |
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Authors: | Xie Can Zhang Jin-Song Zhou Hua-Lin Li Jian Zhang Zhi-Gang Wang Dao-Wen Chen Shou-Yi |
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Institution: | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, Peoples Republic of China. |
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Abstract: | A histidine kinase-based signaling system has been proposed to function in ethylene signal transduction pathway of plants and one ethylene receptor has been found to possess His kinase activity. Here we demonstrate that a His kinase-like ethylene receptor homologue NTHK1 from tobacco has serine/threonine (Ser/Thr) kinase activity, but no His kinase activity. Evidence obtained by analyzing acid/base stability, phosphoamino acid and substrate specificity of the phosphorylated kinase domain, supports this conclusion. In addition, mutation of the presumptive phosphorylation site His (H378) to Gln did not affect the kinase activity whereas deletion of the ATP-binding domain eliminated it, indicating that the conserved His (H378) is not required for the kinase activity and this activity is intrinsic to the NTHK1-KD. Moreover, confocal analysis of NTHK1 expression in insect cells and plant cells suggested the plasma membrane localization of the NTHK1 protein. Thus, NTHK1 may represent a distinct Ser/Thr kinase-type ethylene receptor and function in an alternative mechanism for ethylene signal transduction. |
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Keywords: | two-component kinase Ser Thr kinase ethylene receptor NTHK1 plasma membrane localization tobacco |
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