Callus Culture of Sainfoin (Onobrychis viciifolia) and Plant Regeneration through Somatic Embryogenesis |
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Authors: | GU ZHUPING |
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Institution: | Department of Developmental and Cell Biology, University of California Irvine, CA 92717, USA |
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Abstract: | Callus of sainfoin (Onobrychis viciifolia Scop.) was initiatedfrom stem and root explants which were obtained from seedlingsgrowing in vitro, on Linsmaier Skoog (LS) medium supplementedwith 1 mg l1 2, 4-D and 1 mg l1 BA or only 1 mgl1 BA, and the Vacin and Went medium without hormones.Somatic embryos were formed on LS medium containing 1 m l1BA. Embryos developed into complete plants on filter paper saturatedwith hormone-free LS medium. Onobrychis viciifolia, somatic embryogenesis, callus culture, plant regeneration |
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