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Engineering greater aluminium resistance in wheat by over-expressing TaALMT1
Authors:Jorge F Pereira  Gaofeng Zhou  Emmanuel Delhaize  Terese Richardson  Meixue Zhou  Peter R Ryan
Institution:1Embrapa Trigo, Rodovia BR 285 km 294, CEP 99001-970, Passo Fundo, RS, Brazil;2CSIRO Plant Industry, GPO 1600, Canberra, ACT 2601, Australia;3TIAR, University of Tasmania, PO Box 46, Kings Meadows, Launceston, Tas 7249, Australia
Abstract:MethodsParticle bombardment was used to transform wheat with TaALMT1, the Al3+ resistance gene from wheat, using the maize ubiquitin promoter to drive expression. TaALMT1 expression, malate efflux and Al3+ resistance were measured in the T1 and T2 lines and compared with the parental line and an Al3+-resistant reference genotype, ET8.ConclusionsThe Al3+ resistance of wheat was increased by enhancing TaALMT1 expression with biotechnology. This is the first report of a major food crop being stably transformed for greater Al3+ resistance. Transgenic strategies provide options for increasing food supply on acid soils.
Keywords:Acid soil  aluminium resistance  TaALMT1 gene  transgenic wheat  Triticum aestivum  aluminum
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