Engineering greater aluminium resistance in wheat by over-expressing TaALMT1 |
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Authors: | Jorge F Pereira Gaofeng Zhou Emmanuel Delhaize Terese Richardson Meixue Zhou Peter R Ryan |
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Institution: | 1Embrapa Trigo, Rodovia BR 285 km 294, CEP 99001-970, Passo Fundo, RS, Brazil;2CSIRO Plant Industry, GPO 1600, Canberra, ACT 2601, Australia;3TIAR, University of Tasmania, PO Box 46, Kings Meadows, Launceston, Tas 7249, Australia |
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Abstract: | MethodsParticle bombardment was used to transform wheat with TaALMT1, the Al3+ resistance gene from wheat, using the maize ubiquitin promoter to drive expression. TaALMT1 expression, malate efflux and Al3+ resistance were measured in the T1 and T2 lines and compared with the parental line and an Al3+-resistant reference genotype, ET8.ConclusionsThe Al3+ resistance of wheat was increased by enhancing TaALMT1 expression with biotechnology. This is the first report of a major food crop being stably transformed for greater Al3+ resistance. Transgenic strategies provide options for increasing food supply on acid soils. |
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Keywords: | Acid soil aluminium resistance TaALMT1 gene transgenic wheat Triticum aestivum aluminum |
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