Recovery of Totipotent Cells and Plantlet Production from Daylily Protoplasts

The release of protoplasts by means of enzymes from a totipotentcell suspension of a diploid daylily (Hemerocallis cv. ‘AutumnBlaze’), their collection and distribution in plasticculture vessels and their subsequent regeneration is described.Attention is given to aggregation and to the different formsof growth during the initial stages of culture. The methodsfor the subsequent multiplication of the regenerated cells intomorphogenetically competent compact clusters and the manipulationof these clusters to yield embryo-plantlets are also outlined.The implications of all this in terms of potential for the geneticmodification of daylily is discussed. Hemerocallis, cloning, protoplasts