Adventitious Shoot Regeneration in Carnation (Dianthus caryophyllus) from Axillary Bud Explants |
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Authors: | MILLER ROBYN M; KAUL VIJAY; HUTCHINSON JAMES F; RICHARDS DENNIS |
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Institution: | Horticultural Research Institute, Knoxfield, Department of Agriculture and Rural Affairs P.O. Box 174, Femtree Gully, Victoria, 3156, Australia |
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Abstract: | Adventitious shoots were regenerated from axillary bud explantsof 15 carnation cultivars. The use of leaf and stem explantswas not successful, largely due to explant senescence in thepresence of benzyladenine, kinetin and, to a lesser extent,zeatin. For axillary bud explants, a suitable optimum adventitiousregeneration medium contained Murashige and Skoog basal mediumsolidified with Gelrite and supplemented with 15 µm benzyladenineand 0.5 µM a-napthaleneacetic acid. Adventitious primordiaarose from the cut basal end of bud explants erupting as individualshoots after 23 weeks incubation. The axillary bud sizeand the time between subcultures of source material influencedthe production of adventitious shoots. Transfer of regeneratedshoots onto a medium solidified with agar minimized visiblesigns of vitrification. Regenerated shoots could be easily rooted,transferred to glasshouse conditions and grown to flowering. Vitrification, tissue culture, cut flowers, Dianthus caryophyllus L., carnation, cytokinins, explant |
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