Kinetics and collagenolytic role of eosinophils in chronic gastric ulcer in the rat |
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Authors: | N Ohmiya Shinsuke Saga Masaharu Ohbayashi Ken-ichi Kozaki Osamu Miyaishi Miya Kobayashi Shiro Kasuya Tomiyasu Arisawa Hidemi Goto Tetsuo Hayakawa |
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Institution: | (1) Second Department of Internal Medicine, Nagoya University School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466, Japan Tel. +81 52 744 2172; Fax +81 52 744 2175, JP;(2) Department of Morphology, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Japan, JP;(3) Photon Medical Research Center, Hamamatsu University School of Medicine, Hamamatsu, Japan, JP;(4) Pathophysiology Unit, Aichi Cancer Center Research Institute, Nagoya, Japan, JP;(5) National Institute of Longevity Science, Ohbu, Japan, JP;(6) Second Department of Anatomy, Nagoya University School of Medicine, Nagoya, Japan, JP;(7) Department of Parasitology, Gifu University School of Medicine, Gifu, Japan, JP |
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Abstract: | An association between eosinophils and tissue damage has been observed in numerous disorders. However, few reports have addressed
the role of infiltrating eosinophils in gastric ulcer healing. The aim of this study was to investigate the kinetics and role
of eosinophils infiltrating experimental chronic gastric ulcers in the rat. We developed a monoclonal antibody against human
matrix metalloproteinase 1 (MMP1) purified from conditioned culture medium of human skin fibroblasts. Acetic acid-induced
gastric ulcers were resected from rats on days 1, 3, 5, 10, 20, 40, and 180 after the days of induction (day 0). Tissue specimens
were immunostained with this antibody and examined with an electron microscope. Few eosinophils were observed in the granulation
tissue until day 20. By days 40 and 180, MMP1-positive eosinophils had increased in the granulation tissue of open ulcers.
Azan staining revealed dispersed collagen fibers around infiltrating eosinophils. In contrast, scars demonstrated few eosinophils
in fibrous tissue on days 40 and 180. Eosinophils which express MMP1 infiltrate granulation tissue at the chronic stage of
gastric ulceration. The results suggest that eosinophils may play a role in tissue remodeling and deterioration of ulceration.
Accepted: 18 March 1997 |
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