Attachment of HeLa cells during early G1 phase |
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Authors: | Maarten J A Moes Jose J Bijvelt Johannes Boonstra |
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Institution: | (1) Department of Cell Biology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands; |
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Abstract: | Both growth factor directed and integrin dependent signal transduction were shown to take place directly after completion
of mitosis. The local activation of these signal transduction cascades was investigated in early G1 cells. Interestingly,
various key signal transduction proteins were found in blebs at the cell membrane within 30 min after mitosis. These membrane
blebs appeared in round, mitotic-like cells and disappeared rapidly during spreading of the cells in G1 phase. In addition
to tyrosine-phosphorylated proteins, the blebs contained also phosphorylated FAK and phosphorylated MAP kinase. The formation
of membrane blebs in round, mitotic cells before cell spreading is not specific for mitotic cells, because similar features
were observed in trypsinized cells. Just before cell spreading also these cells exhibited membrane blebs containing active
signal transduction proteins. Inhibition of signal transduction did not affect membrane bleb formation, suggesting that the
membrane blebs were formed independent of signal transduction. |
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Keywords: | |
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