Immunoenzymehistochemical detection of fibrin microthrombi during disseminated intravascular coagulation in rats |
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| Authors: | H Craane J J Emeis J Lindeman W Nieuwenhuizen |
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| Institution: | (1) Department of Pathology, University Medical Centre, Wassenaarseweg 62, Leiden, The Netherlands;(2) Gaubius Institute, Health Research Organization TNO, Herenstraat 5d, Leiden, The Netherlands |
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| Abstract: | Summary In tissue of rats with disseminated intravascular coagulation, fibrin microthrombi can be sensitively detected by immunohistochemical methods, using antisera against rat fibrinogen or fibrin monomer. An indirect immunoperoxidase procedure on paraplast-embedded sections yields best results with regard to the morphology of the thrombi and their localization in the tissue.Only fibrillar immunoreactive material, oriented lengthwise in the vessels, should be regarded as microthrombi formed in vivo. |
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