Human LPLUNC1 is a secreted product of goblet cells and minor glands of the respiratory and upper aerodigestive tracts |
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Authors: | Colin D Bingle Kirsty Wilson Hayley Lunn Frances A Barnes Alec S High William A Wallace Doris Rassl Michael A Campos Manuel Ribeiro Lynne Bingle |
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Institution: | (1) Academic Unit of Respiratory Medicine, Department of Infection and Immunity, University of Sheffield, Sheffield, S10 2JF, UK;(2) Oral and Maxillofacial Pathology, Department of Clinical Dentistry, University of Sheffield, Sheffield, UK;(3) Diagnostic Services Department, Level 6 Medical and Dental School, University of Leeds, Leeds, UK;(4) Department of Pathology, University of Edinburgh, Edinburgh, UK;(5) Department of Pathology, Papworth Hospital, Cambridge, UK;(6) Division of Pulmonary and Critical Care Medicine, University of Miami, Miami, FL, USA |
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Abstract: | Long PLUNC1 (LPLUNC1, C20orf114) is a member of a family of poorly described proteins (PLUNCS) expressed in the upper respiratory
tract and oral cavity, which may function in host defence. Although it is one of the most highly expressed genes in the upper
airways and has been identified in sputum and nasal secretions by proteomic studies, localisation of LPLUNC1 protein has not
yet been described. We developed affinity purified antibodies and localised the protein in tissues of the human respiratory
tract, oro- and nasopharynx. We have complemented these studies with analysis of LPLUNC1 expression in primary human lung
cell cultures and used Western blotting to study the protein in cell culture secretions and in BAL. LPLUNC1 is a product of
a population of goblet cells in the airway epithelium and nasal passages and is also present in airway submucosal glands and
minor glands of the oral and nasal cavities. The protein is not expressed in peripheral lung epithelial cells. LPLUNC1 is
present in bronchoalveolar lavage fluid as two glycosylated isoforms and primary airway epithelial cells produce identical
proteins as they undergo mucociliary differentiation. Our results suggest that LPLUNC1 is an abundant, secreted product of
goblet cells and minor mucosal glands of the respiratory tract and oral cavity and suggest that the protein functions in the
complex milieu that protects the mucosal surfaces in these locations. |
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