Fluorescence methods for the histochemical demonstration of monoamines |
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Authors: | Gösta Jonsson |
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Institution: | (1) Department of Histology, Karolinska Institutet, Stockholm, Sweden;(2) Department of Pharmacology, University of Göteborg, Sweden |
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Abstract: | Summary The histochemical fluorescence method of Falck and Hillarp for the demonstration of catecholamines and certain tryptamines, e.g. 5-hydroxytryptamine is based on the principle that these amines can be condensed with formaldehyde to yield strongly fluorescent 6,7-dihydroxy-3,4-dihydroisoquinolines and 6-hydroxy-3,4-dihydro--carbolines respectively. The investigation here reported presents the fluorescence characteristics and relative fluorescence yields for formaldehyde treated biogenic monoamines and certain related compounds enclosed in a dried protein layer. The fluorescence properties of some synthetic 6,7-substituted-3,4-dihydroisoquinolines and 3,4-dihydro--carbolines are given, and the fluorescence characteristics in relation to the molecular structure are discussed.Abbreviations used A
adrenaline
- DA
dopamine
- DOPA
3,4-dihydroxyphenylalanine
- DOPS
3,4-dihydroxyphenyl-serine
- 5-HT
5-hydroxytryptamine
- 5-HTP
5-hydroxytryptophan
- 5-MT
5-methoxytryptamine
- -m-DA
-methyl-dopamine
- -m-DOPA
-methyl-3,4-dihydroxyphenylalanine
- -m-NA
-methyl-noradrenahne
- MTA
3-methoxy-tyramine
- NA
noradrenaline
- NM
normetanephrine
- T
Tryptamine
- Try
Tryptophan |
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