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Establishment of an effective TLC bioautographic method for the detection of Mycobacterium tuberculosis H37Ra phosphoglucose isomerase inhibition by phosphoenolpyruvate |
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| Authors: | Katarzyna Paradowska Beata Polak Adam Chomicki Grażyna Ginalska |
| Institution: | 1. Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Lublin, Poland and katarzyna.paradowska@umlub.pl;3. Department of Physical Chemistry, Medical University of Lublin, Lublin, Poland;4. Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Lublin, Poland and |
| Abstract: | A bioautographic assay based on thin layer chromatography was developed for phosphoenolpyruvate (PEP) detecting as a known but rarely studied inhibitor of phosphoglucose isomerase (PGI). The protocol with NADP+/NBT/PMS (β-nicotinamide adenine dinucleotide phosphate/nitrotetrazolium blue chloride/phenazine methosulfate) staining was capable of detecting Mycobacterium tuberculosis H37Ra PGI inhibition using PEP. According to this method, visibly brighter spots (zones) against purple background are observed in the area of inhibition of the above-mentioned enzyme activity. The detection limit for PEP as an inhibitor of Mycobacterium tuberculosis H37Ra PGI was 226?μg per spot/zone. Noteworthy is that we are the first authors to have successfully used a bioautographic assay to detect Mycobacterium tuberculosis H37Ra PGI inhibition by PEP. |
| Keywords: | Antimycobacterial activity autography glycolysis mycobacterial cell wall potential drug target |