| 实时PCR技术在植物研究上的应用 |
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| 引用本文： | 童汉华,邬亚文,章善庆,曹一平,罗利军.实时PCR技术在植物研究上的应用[J].中国生物工程杂志,2005,25(5):15-21. |
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| 作者姓名： | 童汉华 邬亚文 章善庆 曹一平 罗利军 |
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| 作者单位： | 1. 中国水稻研究所 杭州 310006;
2. 上海市农业生物基因中心 上海 201106 |
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| 基金项目： | 国家重点基础研究发展计划项目 (G19980 10 2 0 4)，浙江省自然科学基金项目 (M3 0 3 3 87) |
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| 摘 要： | 实时PCR是在常规PCR基础上运用荧光共振能量转移现象,加入荧光标记探针,巧妙地把核酸扩增、杂交、光谱分析和实时检测技术结合在一起的一项新技术,具有快速、灵敏、特异性强、定量准确等特点,广泛应用于医学、检验检疫、军事、农业、基础研究等领域。着重就实时PCR技术的特性及在植物上的应用进行了讨论,并与目前常用的相关技术进行了比较。
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| 关 键 词： | 实时PCR 定量 荧光探针 |
| 修稿时间： | 2004年11月19 |
Application of Real-Time PCR to Plant Study |
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| TONG Han-hua,WU Ya-wen,ZHANG Shan-qing,CAO Yi-ping,LUO Li-jun.Application of Real-Time PCR to Plant Study[J].China Biotechnology,2005,25(5):15-21. |
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| Authors: | TONG Han-hua WU Ya-wen ZHANG Shan-qing CAO Yi-ping LUO Li-jun |
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| Institution: | TONG Han-hua 1,2 WU Ya-wen1 ZHANG Shan-qing1 CAO Yi-ping1 LUO Li-jun2 |
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| Abstract: | Real-time PCR differs from classical PCR in the measurement of the amplified PCR product at each cycle throughout the PCR reaction. It allows the detection of a given nucleic acid target in a rapid, specific and very sensitive way, as well as it affords the absolute quantification of the initial target. Up to date, it is widely used in the domain of medicine, inspection and quarantine, military affairs, agriculture and foundation study. The technical features of quantitative real-time PCR that are especially relevant to plant research were described, and its present and future application were summarized. The technical qualities of real-time PCR, compared with the other currently used techniques, are also discussed. |
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| Keywords: | Real-time PCR Quantification Fluorescent probe |
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