| 洞庭湖四种黄颡鱼基因组DNA遗传多样性的RAPD分析 |
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| 引用本文： | 肖调义,张学文,章怀云,唐湘北,苏建明,刘臻.洞庭湖四种黄颡鱼基因组DNA遗传多样性的RAPD分析[J].中国生物工程杂志,2004,24(3):84-89. |
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| 作者姓名： | 肖调义 张学文 章怀云 唐湘北 苏建明 刘臻 |
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| 作者单位： | 1. 湖南农业大学动物科技学院, 长沙 410128;
2. 湖南农业大学理学院, 长沙 410128;
3. 中南林学院, 长沙 410081 |
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| 基金项目： | 湖南省科技厅资助项目 (0 0NQY10 0 9 2 )，湖南省自然基金资助项目 (0 0JJY2 0 2 1) |
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| 摘 要： | 以洞庭湖瓦氏黄颡鱼、光泽黄颡鱼、长须黄颡鱼、普通黄颡鱼的基因组DNA为材料对 4种黄颡鱼进行了遗传多样性随机扩增多态性DNA(RAPD)分析。通过筛选的 90个引物对 4种黄颡鱼基因组DNA的扩增 ,获得了 36个有效引物 ,并计算出了 4种黄颡鱼群体间的遗传相似系数和遗传距离 ,遗传距离最大的为普通黄颡鱼和瓦氏黄颡鱼 (D =0 9895 ) ,遗传距离最小的为普通黄颡鱼和光泽黄颡鱼 (D =0 672 0 )。同时运用聚类分析 (UPGMA)的方法建立了 4种黄颡鱼的聚类图。
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| 关 键 词： | 黄颡鱼 基因组DNA 遗传多样性 RAPD |
| 收稿时间： | 2004-02-13 |
| 修稿时间： | 2004年2月13日 |
RAPD Analysis of Genetic Diversity on Genomic DNA among 4 Species of Pelteobagrus of Dongting Lake |
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| XIAO Tiao,yiZHANG Xue,wen ZHANG Huai,yun TANG Xiang,bei SU Jian,ming LIU,Zhen\.RAPD Analysis of Genetic Diversity on Genomic DNA among 4 Species of Pelteobagrus of Dongting Lake[J].China Biotechnology,2004,24(3):84-89. |
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| Authors: | XIAO Tiao yiZHANG Xue wen ZHANG Huai yun TANG Xiang bei SU Jian ming LIU Zhen\ |
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| Institution: | XIAO Tiao yi+1ZHANG Xue wen+2 ZHANG Huai yun TANG Xiang bei1 SU Jian ming1 LIU Zhen\1 |
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| Abstract: | Method of RAPD was taken to analysis the genetic diversity on genomic DNA among Pelteobagrus eupogon, Pelteobagrus fulvidraco, Pelteobagrus vachelli and Pelteobagrus nitidus of Dongting Lake. 36 primers were selected from 92 primers. The similarity index and the genetic distances of the four Pelteobagrus were figured out. The largest genetics distances presented between Pelteobagrus and Pelteobagrus eupogon is 0 9895 The least genetics distances presented between Pelteobagrus and Pelteobagrus fulvidraco is 0 6720 Meanwhile, cluster analysis among 4 kinds of Pelteobagrus based on the RAPD results were built by UPGMA method. |
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| Keywords: | Pelteobagrus Genomes DNA Genetic diversity RAPD |
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