三叶半夏组培快繁与优良单株的选育

本文利用组织培养技术实现了三种不同叶型三叶半夏的快速繁殖，并通过光合生理特征的比较对其优良单株进行了选育。结果表明：半夏能在分化培养基MS+6-BA 1.0 mg/L + NAA 0.2 mg/L+蔗糖3%+琼脂5.0 g/L和离体块茎诱导培养基（1/2 MS+IBA 0.03 mg/L+ NAA 0.01 mg/L+蔗糖5%+琼脂5.0 g/L+AC 0.3 g/L）上快速扩繁，继代繁殖系数为1:6.23，培养3个月后块茎的平均直径达0.88cm。 对其光合生理特征的研究结果表明：三叶半夏是一种典型的阴生植物，其光饱和点为700μmol·photons/s·m2；三种叶型的三叶半夏都存在着明显的光抑制现象，其中柳叶型最明显。本研究所选育的11个单株后代中，以桃叶2号（T2）的净光合速率和产量最高，柳叶2号（L2）对强光的耐受力最强。经过6个月的扩繁，本研究获得优良单株T2和L2离体块茎各1万颗。在温州乐清实验基地中经过1年的大田种植后得优良单株半夏块茎各100kg。

Mass Production of Pinellia ternate by tissue culture and the selection for high-yield indivadual

The differences of photosynthetic characters and yields among three leaf-types of Pinallia ternate, the willow-leaf type, the peach-leaf type and the heart-leaf style, were studied and compared. The result showed that P. ternata was a typical shade-plant, of which LCP was only 700μmol?photons/s?m-2. Photo-inhibition was observed among all the three leaf-types of P. ternata, and the willow leaf type showed the most obvious photo-inhibition. Both of the light response Pn curve and single tuber weight were significant different among leaf styles. Result for studying the chlorophyll fluorescence parameters showed that all the difference of NPQ, qP and qN among three leaf-types of P. ternata were significant and the difference of potential-photosynthetic productivity among them was significant. The willow leaf style had the strongest light-tolerance capacity. We found that individuals of T2(peach-leaf type) had the highest Pn and single tuber weight among 11 superior individuals and the individuals of L2(willow-leaf type) showed the strongest light-tolerance capacity.