H2O2对热带假丝酵母醇氧化酶的影响及其作用机理分析

考察了H2 O2 对热带假丝酵母代谢烷烃生产二元酸过程中醇氧化酶 (fattyalcoholoxidase ,AOX)的影响。研究表明 :2mmol L和 5mmol L的H2 O2 对AOX的比酶活有明显的促进作用 ,分别比对照提高了 68 2 %和 82 9%。研究还发现 ,H2 O2 是以超氧阴离子自由基等形式在胞内存在 ,并对H2 O2 在代谢过程中的作用机理进行了分析。

Effect of H2O2 Addition on Alcohol Oxidase in Candida tropicalis and Its Mechanism

AOX is a key enzyme in metabolism of Candida tropicalis. When Candida tropicalis was cultivated in shaking\|flask with the H\-2O\-2 addition, the AOX activity assays indicated that H\-2O\-2 significantly increased the activity of AOX. The specific activity increased by 68\^2% and 82\^9% at 2mmol/L and 5mmol/L H\-2O\-2 addition, respectively. It was also confirmed that H\-2O\-2 may exist as forms of ROS (reactive oxygen species, ROS), such as the generated superoxide radical (O\ -\-2\5). The mechanism of H\-2O\-2 in the metabolism was also discussed.;