内含肽介导PHB纯化人源抗菌肽LL-37体系的构建

新颖的内含肽介导PHB纯化蛋白体系，是一种高效表达、自动切割、纯化方便、费用低廉的蛋白表达纯化体系，有利于蛋白规模化纯化。本研究选用对原核细胞具有毒害作用的小肽--人源抗菌肽LL-37作为纯化对象，通过基因工程技术，构建内含肽介导PHB纯化人源抗菌肽LL-37体系，并利用该体系纯化LL-37。研究结果表明，本研究构建的内含肽介导PHB纯化人源抗菌肽LL-37体系可高效表达LL-37融合蛋白，利用构建的纯化体系能对目的蛋白进行纯化。

System of intein-mediated PHB purified human antimicrobial peptide LL-37

The new intein-mediated PHB purify protein system is a high expression, automatic cutting, for purification, low-cost protein purification system,it is conducive to large-scale protein purification. This research choose human antibacterial peptide LL-37 as the purification objects,which is poison to prokaryotic cell.We construct intein-mediated PHB purified human antimicrobial peptide LL-37 system through genetic engineering technology and use this system to purify LL-37. The results show that this system can highly express LL-37 fusion protein and purifiy the product as same size with expectations