| 还原六价铬细菌及其还原酶的研究 |
| |
| 引用本文： | 魏斐,杨丽荣,薛保国,吴坤.还原六价铬细菌及其还原酶的研究[J].中国生物工程杂志,2012,32(4):53-59. |
| |
| 作者姓名： | 魏斐 杨丽荣 薛保国 吴坤 |
| |
| 作者单位： | 1. 河南省农科院植物保护研究所 河南省农作物病虫害防治重点试验室 郑州 450002;
2. 河南农业大学生命科学学院 郑州 450002 |
| |
| 基金项目： | 河南省超级产粮大省奖励资金资助项目(2001-196-31) |
| |
| 摘 要： | 从活性污泥中筛选出的C-2苏云金芽孢杆菌,能耐受250mg/L的六价铬,并具有较好的还原能力。研究表明木糖、果糖、玉米饼粉、苹果酸、琥珀酸、柠檬酸及Cu2+、Fe2+、Ca2+离子对C-2菌的还原有积极作用,菌体的接种量影响还原的速率。C-2菌还原的最适温度为37℃,最适pH为9.0;六价铬还原酶的最适pH为7.0、温度为37℃,Co2+、Cu2+、Fe2+、DTT、NADH对酶的还原有积极影响。
|
| 关 键 词： | C-2菌 六价铬 还原酶 |
| 收稿时间： | 2011-12-12 |
Study on the Restore Hexavalent Chromium Bacteria and Its Reductase |
| |
| WEI Fei
,
YANG Li-rong
,
XUE Bao-guo
,
WU Kun.Study on the Restore Hexavalent Chromium Bacteria and Its Reductase[J].China Biotechnology,2012,32(4):53-59. |
| |
| Authors: | WEI Fei YANG Li-rong XUE Bao-guo WU Kun |
| |
| Institution: | 1 Institute of Plant Protection,Henan Academy of Agricultural Sciences,Crop Pest and Disease Control in Henan Province Key Laboratory,Zhengzhou 450002,China)(2 College of Life Science,Henan Agricultural University,Zhengzhou 450002,China) |
| |
| Abstract: | The Bacillus thuringiensis C-2 strain was isolated from activated sludge and it can tolerant 250mg/L Cr6+ and have better reducing capacity.The results showed that xylose,fructose,corn cake powder,malic acid,succinic acid,citric acid and ions Cu2+、Fe2+、Ca2+ had an active role for reduction and the inoculum size had influence for reduction.The conditions of 37℃ and pH9.0 were good for C-2 strain to reduce the Cr6+.The optimum pH and temperature of chromium reductase was pH7.0 and 37℃.The Co2+,Cu2+,Fe2+,DTT,NADH effectively promoted the reduction. |
| |
| Keywords: | C-2 strain Hexavalent chromium Reductase |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国生物工程杂志》浏览原始摘要信息 |
| 点击此处可从《中国生物工程杂志》下载免费的PDF全文 |
|
