| 新型小片段基因表达载体构建方法 |
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| 引用本文： | 金磊,赵文秀,马岚.新型小片段基因表达载体构建方法[J].中国生物工程杂志,2012,32(6):57-63. |
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| 作者姓名： | 金磊 赵文秀 马岚 |
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| 作者单位： | 1. 清华大学生命科学学院 北京 100084;2. 清华大学深圳研究生院生命与健康学部 深圳 518055 |
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| 基金项目： | 国家“863”计划资助项目(2006AA03Z359) |
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| 摘 要： | 介绍一种新型快速构建小片段基因表达载体的方法。该方法省去了目的基因酶切和酶切后纯化步骤,并省去了载体酶切后胶回收纯化步骤,具有简单快速,节省试剂费用,连接效率高等特点。应用该方法已经完成了4个小片段基因(67bp)表达载体的构建。
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| 关 键 词： | 小片段 表达载体 有效碰撞 |
| 收稿时间： | 2012-02-07 |
A Novel Method for Constructing Small Fragment Gene of Expression Vector |
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| JIN Lei
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ZHAO Wen-xiu
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MA Lan.A Novel Method for Constructing Small Fragment Gene of Expression Vector[J].China Biotechnology,2012,32(6):57-63. |
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| Authors: | JIN Lei ZHAO Wen-xiu MA Lan |
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| Institution: | 1 School of Life Sciences,Tsinghua University,Beijing 100084,China)(2 Division of Life Science & Health,Graduate School at Shenzhen,Tsinghua University,Shenzhen 518055,China) |
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| Abstract: | A new method for rapid constructing small fragment of expression vector is introduced.This method eliminates the need for the target gene digested with restriction enzyme and recovery steps,and eliminates the vector gel purified steps after digestion.It is simple and fast,saving reagent costs,high connection efficiency.Four expression vectors of small fragment gene(67bp) have been completed with this method. |
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| Keywords: | Small fragment Expression vector Effective collision |
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