| siRNA脱靶效应类型与规避策略 |
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| 引用本文： | 唐德平,毛爱红,廖世奇,薛林贵,张柄林.siRNA脱靶效应类型与规避策略[J].中国生物工程杂志,2012,32(7):113-119. |
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| 作者姓名： | 唐德平 毛爱红 廖世奇 薛林贵 张柄林 |
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| 作者单位： | 1. 兰州交通大学化学与生物工程学院 兰州 730070;2. 甘肃省医学科学研究院 兰州 730050 |
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| 基金项目： | 国家自然科学基金(81060180);甘肃省中青年基金(1107RJYA033)资助项目 |
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| 摘 要： | 小干扰RNAs(small interfere RNAs,siRNAs)能够特异性沉默靶基因,现已广泛应用于阐明基因功能,鉴定药物靶点,开发比目前更有效的治疗药物。然而siRNA脱靶效应(off-target effects,OTEs)导致基因沉默实验中表型效应解释复杂化,引起siRNA治疗毒副作用。与siRNA有关的脱靶效应有microRNA样脱靶效应、免疫刺激、RNAi元件饱和三种类型。综述了siRNA脱靶效应类型及减轻脱靶效应的方法,以增强该技术的实用性。
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| 关 键 词： | RNA干扰 小干扰RNA 脱靶效应 microRNA样脱靶效应 |
| 收稿时间： | 2012-02-21 |
The Types of siRNA Off-target Effects and the Strategies for Mitigation |
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| TANG De-ping
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MAO Ai-hong
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LIAO Shi-qi
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XUE Lin-gui
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ZHANG Bing-lin.The Types of siRNA Off-target Effects and the Strategies for Mitigation[J].China Biotechnology,2012,32(7):113-119. |
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| Authors: | TANG De-ping MAO Ai-hong LIAO Shi-qi XUE Lin-gui ZHANG Bing-lin |
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| Institution: | 1(1 The School of Chemical & Biological Engineering,Lanzhou Jiaotong University,lanzhou 730070,China)(2 Institute of Gansu Medical Science Research,lanzhou 730050,China) |
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| Abstract: | Small interfering RNAs(siRNAs) can specific silence target genes,and are widely used to elucidate gene function,indentify drug targets and develop more specific therapeutics than are currently available.Off-target effects(OTEs) can complicate the interpretation of phenotypic effects in gene-silencing experiments and can potentially lead to unwanted toxicities.siRNAs OTEs include microRNA-like off-target effects,immune stimulation and saturation of the RNAi machinery.The types of off-target effects of siRNAs and methods to mitigate them was focused,to help enable effective application of RNAi technology. |
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| Keywords: | RNAi siRNA Off-target effects MicroRNA-like off-target effects |
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