快速PCR研究进展

PCR是最常用的分子生物学技术之一，通过变性、退火和延伸的循环来完成核酸分子的大量扩增。快速PCR就是基于普通PCR的工作原理，在保证PCR反应特异性、灵敏性、保真度的前提下，在更短时间内完成对核酸分子的扩增。近年来已经开展了许多有关方面的研究工作。本文将以DNA聚合酶的改进、添加剂的选择、热循环仪改进为重点内容，综述快速PCR技术的研究进展。

The Development of Rapid PCR

Polymerase chain reaction (PCR) is one of the common techniques in molecular biology, which can amplify nucleic acids through the cycle of denaturation, annealing and extension. Based on the principle of common PCR, rapid PCR is to realize the amplification of nucleic acids in less time without affecting the specificity, sensitivity and fidelity of the reaction. A lot of research work in this field has been going on in recent years. This article will make a review of the development of rapid PCR with emphases on the improvement of DNA polymerase, the choice of additives and the improvement of thermocyclers.