包涵体蛋白的分离和色谱法体外复性纯化研究进展

重组蛋白在大肠杆菌中表达多为无活性的包涵体形式，须经洗涤、溶解、复性后才能得到生物活性蛋白。综述了近年来包涵体蛋白分离纯化和复性技术研究进展，重点讨论了色谱法复性技术的应用，包括尺寸排阻色谱、亲和色谱、离子交换色谱、疏水相互作用色谱、固定化脂质体色谱、扩张床吸附色谱的进展情况。

The Progress of Inclusion Body Isolation and Chromatographic Refolding,Purification Methods

Abstract Expression of recombinant proteins in Escherichia coli often results inclusion bodies, an aggregation form of inactive proteins. Functional proteins can only be gained through a series of steps including washing, solubilization and refolding. The inclusion body purification strategy and chromatographic refolding techniques including size exclusion chromatography, affinity chromatography, ion-exchange chromatography, hydrophobic interaction chromatography, immobilized liposome chromatography, and expanded bed adsorption chromatography were summarized in this review.