| 腺病毒载体纤突结的改造及改造后的特性研究 |
| |
| 引用本文： | 乔伟,何金生,付远辉,洪涛.腺病毒载体纤突结的改造及改造后的特性研究[J].中国生物工程杂志,2011,31(6):86-92. |
| |
| 作者姓名： | 乔伟 何金生 付远辉 洪涛 |
| |
| 作者单位： | 1. 安徽医科大学免疫学教研室 合肥 230032;
2. 北京交通大学生命科学与生物工程研究院 北京 100044;
3. 中国疾病预防与控制中心病毒病预防控制所 北京 100052 |
| |
| 摘 要： | 目的:采用一种简便方法在复制缺陷型腺病毒载体纤突结(fibre knob)的HI loop插入精氨酸-甘氨酸-天冬氨酸(arginine-glycine-aspartate,RGD)肽,构建纤突结修饰的腺病毒载体,观察其对RD和T24肿瘤细胞的感染效率以及机体针对外源基因的抗体反应.方法:以PCR方法将RGD插入到pA...
|
| 关 键 词： | 复制缺陷型腺病毒载体 RGD 荧光 抗体反应 |
| 收稿时间： | 2010-11-15 |
| 修稿时间： | 2011-02-17 |
The Characterization of Modified Adenovirus Vector |
| |
| QIAO Wei,HE Jin-shen,FU Yuan-hui,HONG Tao.The Characterization of Modified Adenovirus Vector[J].China Biotechnology,2011,31(6):86-92. |
| |
| Authors: | QIAO Wei HE Jin-shen FU Yuan-hui HONG Tao |
| |
| Institution: | QIAO Wei1,2 HE Jin-shen1,2 FU Yuan-hui2 HONG Tao2,3(1 Department of Immunology,Anhui Medical University,Hefei 230032,China)(2 College of Life Sciences & Bioengineering,Beijing Jiaotong University,Beijing 100044,China)(3 Institute of Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 100052,China) |
| |
| Abstract: | Objective: A simple way to construct an adenovirus vector containing a peptide-RGD in the HI loop of the fiber knob was developed and then its infection efficiency on RD and T24 cells in vitro and the humoral responses to transgenes in vivo were investigated.Methods: The pAdEasy-RGD-1 was modified by inserting RGD motif into pAdEasy-1 based on simple PCR method.Enhanced green fluorescent protein(EGFP) and codon optimized fusion protein(Fsyn) of human respiratory syncytial virus(RSV) were cloned and constuct... |
| |
| Keywords: | Replication-defective adenovirus vector RGD Fluorensence Humoral immune response |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国生物工程杂志》浏览原始摘要信息 |
| 点击此处可从《中国生物工程杂志》下载免费的PDF全文 |
|
