| 壳寡糖缓解甲萘醌诱导巨噬细胞损伤机制初探 |
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| 引用本文： | 麻攀,刘洪涛,许青松,白雪芳,杜昱光.壳寡糖缓解甲萘醌诱导巨噬细胞损伤机制初探[J].中国生物工程杂志,2011,31(6):18-21. |
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| 作者姓名： | 麻攀 刘洪涛 许青松 白雪芳 杜昱光 |
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| 作者单位： | 1. 中国科学院大连化学物理研究所 大连 116023;
2. 中国科学院研究生院 北京 100049 |
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| 基金项目： | 国家科技重大专项课题资助项目(2009ZX09501-011) |
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| 摘 要： | 目的:研究壳寡糖对甲萘醌诱导的巨噬细胞氧化损伤的保护作用.方法:通过MTT实验检测相应处理的细胞活力,并通过相应试剂盒检测细胞氧化还原体系中某些相关酶的活力及相应产物含量.结果:壳寡糖能够缓解甲萘醌诱导的细胞损伤,并且发现壳寡糖可以缓解甲萘醌导致的胞内超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-PX)活力的降...
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| 关 键 词： | 壳寡糖 巨噬细胞 氧化应激 |
| 收稿时间： | 2010-12-20 |
| 修稿时间： | 2011-04-01 |
Effects of Chitosan Oligosaccharides Attenuating Menadione-induced Injury in Macrophages |
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| MA Pan,LIU Hong-tao,XU Qing-song,BAI Xue-fang,DU Yu-guang.Effects of Chitosan Oligosaccharides Attenuating Menadione-induced Injury in Macrophages[J].China Biotechnology,2011,31(6):18-21. |
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| Authors: | MA Pan LIU Hong-tao XU Qing-song BAI Xue-fang DU Yu-guang |
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| Institution: | MA Pan1,2 LIU Hong-tao1,2 XU Qing-song1 BAI Xue-fang1 DU Yu-guang1(1 Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China)(2 Graduate School of Chinese Academy of Sciences,Beijing 100049,China) |
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| Abstract: | Aim: To investigate the effects of Chitosan oligosaccharides(COS) on attenuating menadione-induced stress injury in macrophages.Method: MTT assay was used to examine the cell viability,and the reagent kits were used to investigate the activities of enzymes and the products of lipid peroxidation in redox system.Results: By using MTT assay and detecting the activities of LDH(lactate dehydrogenase) in the cultural supernatants,it was confirmed that chitosan oligosaccharides could attenuate menadione-induced st... |
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| Keywords: | Chitosan oligosaccharides(COS) Macrophage Oxidative stress |
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