| 香蕉乙二醛酶基因增强酿酒酵母对非生物胁迫抵抗能力的研究 |
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| 引用本文： | 邓成菊,张建斌,贾彩红,金志强,徐碧玉.香蕉乙二醛酶基因增强酿酒酵母对非生物胁迫抵抗能力的研究[J].中国生物工程杂志,2010,30(8):22-26. |
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| 作者姓名： | 邓成菊 张建斌 贾彩红 金志强 徐碧玉 |
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| 作者单位： | 1.中国热带农业科学院热带生物技术研究所 海口 571101
2.农业部热带作物生物技术重点开放实验室 海口 571101
3.海南大学农学院 海口 571101
4.中国热带农业科学院海口实验站 海口 571101 |
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| 基金项目： | 公益性行业(农业)科研专项项目，现代农业产业技术体系建设专项项目，中央级公益性科研院所基本科研业务费专项资金项目 |
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| 摘 要： | 从香蕉cDNA文库中克隆到了一个香蕉(Musa acuminata AAA subgroup)乙二醛酶(glyoxalase,GLO)基因(MaGLO14)。构建了带有MaGLO14的酵母表达载体PYES2-MaGLO14,转化酿酒酵母(Saccharomyces cerevisiae)尿嘧啶营养缺陷型菌株INVSC1,挑取转化子进行PCR和酶切鉴定,证实获得了转基因菌株。通过比较转基因菌株和非转基因菌株在NaCl、高温、低温、干旱、UV胁迫下的生长状况,证明转基因菌株在以上非生物胁迫条件下的存活菌落数均高于非转基因菌株。利用酿酒酵母初步证明MaGLO14具有增强酵母菌对非生物胁迫抵抗力的功能。
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| 关 键 词： | 香蕉 乙二醛酶 乙二醛酶基因 非生物胁迫 酿酒酵母 转基因 |
| 收稿时间： | 2010-03-22 |
| 修稿时间： | 2010-05-20 |
Enhancement of Tolerance to Abiotic Stress of Saccharomyces cerevisiae Transformed by a Gene Encoding Glyoxalase from Banana |
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| DENG Cheng-ju,ZHANG Jian-bin,JIA Cai-hong,JIN Zhi-qiang,XU Bi-yu.Enhancement of Tolerance to Abiotic Stress of Saccharomyces cerevisiae Transformed by a Gene Encoding Glyoxalase from Banana[J].China Biotechnology,2010,30(8):22-26. |
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| Authors: | DENG Cheng-ju ZHANG Jian-bin JIA Cai-hong JIN Zhi-qiang XU Bi-yu |
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| Institution: | DENG Cheng-ju1,3 ZHANG Jian-bin1,2 JIA Cai-hong1,2 JIN Zhi-qiang1,2,4,XU Bi-yu1,2 (1 Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Science,Haikou 571101,China) (2 Key Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture,China) (3 Department of Agriculture,Hainan University,China) (4 Chinese Academy of Tropical Agricutural Sciences Haikou Experimental Station,China) |
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| Abstract: | The Glyoxalase(GLO) plays a very important role in plant detoxification system.Glyoxalase gene was isolated from banana (Musa acuminata AAA subgoup)cDNA library,named as MaGLO14. To confirm the tolerance of MaGLO14 to abiotic stresses,a yeast expression vector PYES2-MaGLO14 has been constructed and transformed into Saccharomyces cerevisiae strain INVSC1 with uracil auxotrophic phenotype. Comparison of colony numbers of transgenic INVSC1 with non-transgenic INVSC1 grown in medium containing NaCl,under high t... |
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| Keywords: | Banana (Musa acuminata AAA subgoup) Glyoxalase Glyoxalase gene Abiotic stress Yeast (Saccharomyces cerevisiae) Transgenic yeast |
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