重组大肠杆菌高密度发酵研究进展

重组大肠杆菌的高密度发酵是提高基因工程产品产量的一个非常有效的手段,是现代发酵工程研究的一个热点。本文就高密度发酵中影响重组大肠杆菌发酵产率的几个因素,包括宿主菌、培养基、培养条件、补料方法以及高密度发酵过程中存在的问题和对策加以讨论,着重探讨了高密度下大肠杆菌产生的有害代谢副产物———乙酸的产生机制、抑制作用机理,以及控制乙酸积累的技术方法 。

Progress Studies of High Cell-density Culture of Recombinant Escherichia coli

High cell\|density fermentation of recombinant E.coli was an efficient approach to prove production yield and a hot point in fermentation engineering.This article discussed several factors influencing the productivity including host,medium,cultivation conditions,feeding strategies,together with the problems and solutions in high cell\|density fermentation of recombinant E.coli .The mechanism of production,inhibition,and techniques of limitation accumulation of acetic acid were emphasized,which was a major inhibitor of cell growth and reduction of productivity among the bacterial metabolic byproducts in high cell density fermentation.