| 重组大肠杆菌产Streptomyces sp. FA1来源木聚糖酶的摇瓶发酵优化 |
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| 引用本文： | 何洁,宿玲恰,吴敬.重组大肠杆菌产Streptomyces sp. FA1来源木聚糖酶的摇瓶发酵优化[J].中国生物工程杂志,2013,33(2):41-46. |
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| 作者姓名： | 何洁 宿玲恰 吴敬 |
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| 作者单位： | 1. 江南大学食品科学与技术国家重点实验室 无锡 214122;2. 江南大学生物工程学院工业生物技术教育部重点实验室 无锡 214122 |
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| 基金项目： | 国家自然科学基金(30970057)(111-2-06)、江苏省科学与技术支持项目(BE2012018)资助项目 |
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| 摘 要： | 为了实现来源于Streptomyces sp. FA1的木聚糖酶的高效胞外分泌表达,对E.coli BL21(DE3)/pET20b(+)/coe/xynA基因工程菌的发酵产酶诱导条件进行优化,获得最优的诱导条件为25 ℃发酵6 h后添加终浓度为0.4 mmol/L的IPTG。在此基础上对发酵培养基进一步优化,得到最优培养基成分为:甘油11 g/L,酵母粉24 g/L,蛋白胨8 g/L,磷酸盐浓度89 mmol/L,镁离子4 mmol/L。最终酶活达到780.2 U/ml,为未优化前的2.2倍,是目前大肠杆菌摇瓶发酵产木聚糖酶的最高表达水平,为实现该酶的工业化生产奠定基础。
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| 关 键 词： | 链霉菌 木聚糖酶 重组大肠杆菌 发酵优化 |
| 收稿时间： | 2012-12-09 |
| 修稿时间： | 2013-01-10 |
Optimization of Fermentation in Shake Flasks for the Xylanase in Recombinant E. coli |
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| HE Jie,SU Ling-qia,WU Jing.Optimization of Fermentation in Shake Flasks for the Xylanase in Recombinant E. coli[J].China Biotechnology,2013,33(2):41-46. |
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| Authors: | HE Jie SU Ling-qia WU Jing |
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| Institution: | 1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;2. School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, Wuxi 214122, China |
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| Abstract: | In order to achieve high production of the xylanase, the optimization of culture medium was investigated in E. coli BL21(DE3) harboring the plasmid pET20b(+)/coe/xynA. The optimized medium and induction condition were as follows: 11 g/L glycerol, 24 g/L yeast extract, 8 g/L peptone, 89 mmol/L PO43-, 4 mmol/L Mg2+, induced by 0.4 mmol/L IPTG at 6h of culture. Under this condition, the enzyme activity increased from 346.8 U/ml to 780.2 U/ml, which was 2.2 times as high as that when it was not optimized. |
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| Keywords: | Streptomyces sp FA1 Xylanase Recombinant E coli Optimization |
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