| 基于2A肽策略构建多基因表达载体的研究进展 |
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| 引用本文： | 张欢,黄思超,蔡绍晖.基于2A肽策略构建多基因表达载体的研究进展[J].中国生物工程杂志,2013,33(1):104-108. |
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| 作者姓名： | 张欢 黄思超 蔡绍晖 |
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| 作者单位： | 1. 暨南大学药学院 广州 510632;2. 珠海市人民医院 珠海 519000 |
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| 基金项目： | 国家自然科学基金资助项目(81273538) |
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| 摘 要： | 多基因表达载体的构建在生物技术领域尤其是基因治疗方面显得日趋重要。目前常用的多基因表达载体多存在载体容量小、上下游基因表达失衡、蛋白活性低或蛋白空间位置不理想等缺陷。2A肽是近年使用较多的一种构建多基因载体的工具,与其它多基因构建策略相比,2A肽策略具有更明显优势。就(类)2A肽的来源、剪切机制、生物学特征、与蛋白定位的关系以及应用方面做一综述。
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| 关 键 词： | 多基因载体 2A肽 共表达 |
| 收稿时间： | 2012-09-04 |
| 修稿时间： | 2012-11-22 |
Development of 2A Peptide-based Strategies for Constructing Multicistronic Expression Vectors |
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| ZHANG Huan,HUANG Si-chao,CAI Shao-hui.Development of 2A Peptide-based Strategies for Constructing Multicistronic Expression Vectors[J].China Biotechnology,2013,33(1):104-108. |
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| Authors: | ZHANG Huan HUANG Si-chao CAI Shao-hui |
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| Institution: | 1(1College of Pharmacy,Jinan University,Guangzhou 510632,China)(2Department of Pharmacy,The People’s Hospital of Zhuhai,Zhuhai 519000,China) |
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| Abstract: | The construction of multicistronic vectors is important for biotechnology, especially for disease therapy. However, there are some limitations of the commonly used vectors such as limited capcity of vectors, imbalance of protein expression, low activity of protein or limited in the same subcellular space. 2A peptide has been used for construction of multicistronic vectors in recent years. Vectors containing 2A peptide definitely outweigh other construction strategies. The resource of 2A peptide, cleave mechanism, biological character, the relationship with protein targeting and application are reviewed. |
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| Keywords: | Multicistronic vectors 2A peptide Co-expression |
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