| 一种简便高效的改良降落PCR |
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| 引用本文： | 王天云,张贵星,薛乐勋.一种简便高效的改良降落PCR[J].中国生物工程杂志,2003,23(11):80-82. |
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| 作者姓名： | 王天云 张贵星 薛乐勋 |
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| 作者单位： | 1. 郑州大学河南省分子医学重点学科开放实验室, 郑州 450052;
2. 新乡医学院细胞生物学教研室新乡453003 |
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| 基金项目： | 国家自然科学基金资助项目 ( 3 0 2 70 0 3 1) |
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| 摘 要： | 降落(touchdown,TD)PCR通常涉及15个退火温度,程序设计复杂。报道了一种简便高效的改良降落PCR,只需5个降落退火温度,以杜氏盐藻(Dunaliellabardawil)基因组为模板,设计一对引物扩增胡萝卜素生物合成相关(carotenebiosynthesisrelated,cbr)基因的第3外显子。实验证实该方法程序简单,比标准降落PCR步骤简化70%,且产物的特异性及效率都有较大提高。
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| 关 键 词： | PCR 降落PCR 特异性 产物得率 |
| 修稿时间： | 2003年7月7日 |
A Simple and Efficient Modified Touchdown PCR |
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| Wang Tianyun , Zhang Guixing Xue Lexun.A Simple and Efficient Modified Touchdown PCR[J].China Biotechnology,2003,23(11):80-82. |
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| Authors: | Wang Tianyun Zhang Guixing Xue Lexun |
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| Institution: | Wang Tianyun 1,2 Zhang Guixing 1 Xue Lexun 1 |
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| Abstract: | Touchdown PCR is a complex program that usually involves fifteen different annealing temperatures.A simple and efficient modified touchdown PCR only involving five different annealing temperatures was described, Dunaliella bardawil genomic DNA was used as template,and a pair of primer was designed to amplify the third exon of carotene biosynthesis related gene.It was demonstrated that the specificity and efficiency of PCR product were significantly improved. |
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| Keywords: | PCR touchodwn PCR Specificity Efficiency of PCR product |
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