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定量测定重组人睫状神经营养因子活性的新方法
引用本文:周虎,汪怡,朱俊铭.定量测定重组人睫状神经营养因子活性的新方法[J].中国生物工程杂志,2008,28(2):86-89.
作者姓名:周虎  汪怡  朱俊铭
作者单位:丝宝集团科研中心生命科学研究所 丝宝集团科研中心生命科学研究所 丝宝集团科研中心生命科学研究所
摘    要:目的:建立一种能定量测定重组人睫状神经营养因子(ciliary neurotrophic factor,CNTF)生物学活性的新方法。方法:从鸡胚中分离出背根神经节并制成神经细胞,将重组人睫状神经营养因子加入到细胞中继续培养64h后,用酸性磷酸酶法检测活细胞内酸性磷酸酶的活性,从而定量测定重组人睫状神经营养因子的生物活性。结果:重组人睫状神经营养因子有促原代鸡胚背根神经细胞存活作用,细胞存活率与加入重组人睫状神经营养因子的量成正相关。结论:通过检测存活的原代鸡胚背根神经细胞内酸性磷酸酶的含量来定量测定重组人睫状神经营养因子生物活性的实验方法具有干扰因素少、定量准确、重复性好等优点。

关 键 词:睫状神经营养因子  酸性磷酸酶  神经细胞  生物学活性  定量测定  重组  人睫状神经营养因子  生物活性  实验方法  Ciliary  Neurotrophic  Factor  Human  Recombinant  Biological  Activity  Determination  Method  干扰因素  含量  酸性磷酸酶  酶法检测  相关  细胞存活率  作用  背根神经节  结果  酶的活性
收稿时间:2007-06-04
修稿时间:2007年6月4日

A New Quantitative Determination Method for The Biological Activity of Recombinant Human Ciliary Neurotrophic Factor
ZHOU Hu,WANG Yi,ZHU Jun-ming.A New Quantitative Determination Method for The Biological Activity of Recombinant Human Ciliary Neurotrophic Factor[J].China Biotechnology,2008,28(2):86-89.
Authors:ZHOU Hu  WANG Yi  ZHU Jun-ming
Abstract:Object: To develop a new quantitative determination method for the biological activity of recombinant human ciliary neurotrophic factor. Methods: Dorsal root ganglions were derived from the chick embryo and dispersed into single neuron cell,The rhCNTF was added to neuron cells and incubated for 64 hours,The activity of acid phosphatase in neuron cells was determined and the biological activity of rhCNTF was analyzed quantificationally. Result: rhCNTF could promote original era dorsal root neuron cells of chick embryo surviving,the livability of neuron cells was positively related to the amount of rhCNTF added to the culture. Conclusion: A quantitative determination method for the biological activity of rhCNTF was developed by testing the activity of acid phosphatase in neuron cells. Compared with the typical ways,this method was quantificational easily,repeatable better and with much fewer disturbance factors.
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