小鼠-牛体细胞种间核移植

本文探讨了小鼠-牛异质胚构建的简便方法及小鼠体细胞核在牛卵母细胞中重新编程的可能性。以牛的卵母细胞为细胞质供体，用去除透明带及徒手切割的方法去核，设定电压1.5 KV/cm，脉冲时间40μsec，与小鼠皮肤成纤维细胞进行电融合的融合率为67.44%，卵裂率为30.23%。融合细胞经离子酶素-6-DMAP激活，用微滴内压制做窝的方法培养小鼠皮肤成纤维细胞异质胚，异质胚的最终发育阶段为8细胞期。结果表明，去透明带牛卵母细胞经切割法去核，可用于小鼠异质胚构建；微滴内做窝的体外培养方法可避免无透明带胚胎的聚合。

Mouse-bovine somatic cell interspecies nuclear transfer

simplified interspecies nuclear transfer in mouse and bovine and the possibilities of the embryos reprogramming were developed in this study. The zona and nucleus of the bovine oocyte were removed by pronase and bisection method respectively. When fibroblast cell of the mouse skin fused with the nucleus-free oocyte of the bovine using 40μsec and 1.5 KV/cm direct current pulse，the rates of fusion and cleavage were 67.44% and 30.23% respectively. Reconstructed heterogenous embryos finally developed to 8-cell stage when they were previously activated by ionomycin and 6-DMAP and subsequently cultured in press-made micro-well in the drop of the medium. Zona-free bisection method could be used for mouse heterogenous embryos cloning, and the micro-well culture method could be used to avoid zona-free embryos aggregation.