促进植物高效遗传转化的发育调节因子及其在玉米中的应用进展*

高效遗传转化技术体系的建立对植物功能基因组学研究和作物新品种的培育均具有促进作用,目前,再生效率低下是限制许多植物高效遗传转化体系建立的主要技术屏障之一。随着对植物分生组织和体细胞胚形成过程研究的深入,鉴定到了一些关键调控基因,统称为发育调节因子。发育调节因子应用于植物遗传转化后,可以有效改善植物分生组织诱导和再生能力,为提高遗传转化效率提供了重要机遇。综述了7类发育调节因子在提高植物遗传转化效率中的研究进展,重点介绍了其中3类在促进玉米遗传转化中的应用,最后展望了建立植物高效遗传转化体系的发展方向。

Development Regulatory Factors Promoting Efficient Plant Genetic Transformation and Their Application in Maize

The establishment of efficient genetic transformation systems can promote the study of plant functional genomics and the cultivation of new crop varieties. At present, low regeneration efficiency is one of the main technical barriers to the establishment of efficient genetic transformation systems in many plants. With the deepening of research on plant meristem and somatic embryo formation, some key regulatory genes have been identified, which are collectively called developmental regulators. The application of developmental regulatory factors in plant genetic transformation can effectively improve the establishment of plant meristem induction and regeneration ability, which provides an important opportunity to improve the efficiency of genetic transformation. In this paper, the research progress of seven types of developmental regulatory factors in improving the efficiency of plant genetic transformation was reviewed, with emphasis on the application of three types of developmental regulatory factors in promoting maize genetic transformation. Finally, the development direction of establishing efficient plant genetic transformation system was presented.