| 玉米类受体蛋白激酶基因ZmLRRPK1的cDNA克隆与表达分析 |
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| 引用本文： | 边鸣镝,邓川,白忠义.玉米类受体蛋白激酶基因ZmLRRPK1的cDNA克隆与表达分析[J].中国生物工程杂志,2009,29(7):33-36. |
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| 作者姓名： | 边鸣镝 邓川 白忠义 |
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| 作者单位： | 吉林大学植物科学学院 |
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| 摘 要： | 富亮氨酸重复区的类受体蛋白激酶(LRR-RLKs)在植物对多种信号的响应中发挥作用。应用电子克隆的方法在玉米中克隆了一个LRR-RLKs类基因的全长cDNA，命名为ZmLRRPK1（GenBank接受号为EU873320）。推断的ZmLRRPK1蛋白含有594个氨基酸，分子量为66kDa，等电点pI为5.42，具有典型的LRR-RLKs结构域。半定量RT-PCR结果表明，在ABA、甘露醇和氯化钠的诱导下，ZmLRRPK1在玉米胚芽鞘中表达并在24 h内保持较高的转录水平。
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| 关 键 词： | 玉米 富亮氨酸重复区的类受体蛋白激酶 信号传导 非生物胁迫 |
| 收稿时间： | 2009-02-27 |
| 修稿时间： | 2009-04-15 |
cDNA Cloning of Maize Protein Kinase Gene ZmLRRPK1 and Its Expression Analysis |
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| BIAN Ming-di,DENG Chuan,BAI Zhong-yi.cDNA Cloning of Maize Protein Kinase Gene ZmLRRPK1 and Its Expression Analysis[J].China Biotechnology,2009,29(7):33-36. |
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| Authors: | BIAN Ming-di DENG Chuan BAI Zhong-yi |
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| Institution: | （College of Plant Science, Jilin University, Changchun130062, China） |
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| Abstract: | Abstract Abstract LRR-RLKs plays an important role in multiple signal transduction in plant. One full-length cDNA encoding a LRR-RLKs homologue was isolated from maize through in silico cloning and named as ZmLRRPK1 ( GenBank accession:EU873320 ). The predicted ZmLRRPK1 protein has 594 amino acids with an estimated molecular mass of 66kDa and an isoelectric point of 5.42. ZmLRRPK1 has the typical domain of LRR-RLKs. RT-PCR analysis indicated that ZmLRRPK1 expression was induced by ABA, mannitol and salt in coleoptiles of maize and kept high level during 24 hours. |
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| Keywords: | Zea mays LRR-RLKs signal transduction abiotic sress |
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