转基因衣藻lba及对照藻产氢培养条件的优化

通过对莱茵衣藻849及其转基因衣藻lba进行光照强度、细胞浓度和培养基中硫酸盐含量三因素三水平的正交实验,确定了两个藻种的最佳产氢条件,同时对转基因藻和849产氢培养条件下的光合放氧速率和pH进行了检测。实验结果表明,在25 ℃下,莱茵衣藻849和转基因衣藻lba的最佳产氢条件都为光照强度 60μmol/(m²·s),细胞浓度为叶绿素含量12.5μg/ml,培养基中硫酸盐含量0μmol/L。莱茵衣藻849和转基因衣藻lba的最高氢气产量分别达到了349μl/mg chlorophyll 和634μl/mg chlorophyll。在产氢条件下,转基因藻lba的净光合放氧速率比849低。结果为利用豆血红蛋白特性通过基因工程手段提高莱茵衣藻产氢量提供基础实验数据。

Optimization of Culture Conditions for Hydrogen Production of Chlamydomonas reinhardtii and its Transgenic Strain lba

In order to determine the optimal culture conditions for hydrogen production of Chlamydomonas reinhardtii strain 849 and its transgenic alga lba, the orthogonal experiments were designed to test the effects of such three factors as light intensity, cell density and the sulfur content in the culture medium at three levels, meanwhile O₂- evolution activity and pH value were detected. The results showed that at 25 ℃, the optimal culture conditions for hydrogen production for both strain 849 and the transgenic alga lba were under 60μmol/(m²·μs) of light intensity, 12.5μg/ml of the chlorophyll content as cell density and 0μmol/L of sulfate content in the medium. Under such condition, the maximal hydrogen yield of strain 849 and the transgenic algae lba reached 349μl/mg chl and 634μl/mg chl respectively. The net O₂- evolution activity of transgenic alga lba was lower than 849.The results provided basic data for the utilization of leghemoglobin in the improvement of hydrogen production of C. reinhardtii with bioengineering methods in the future.