| 二硫键异构酶 |
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| 引用本文： | 熊向华,刘志敏.二硫键异构酶[J].中国生物工程杂志,2005,25(B04):176-179. |
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| 作者姓名： | 熊向华 刘志敏 |
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| 作者单位： | 军事医学科学院生物工程研究所,北京100071 |
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| 基金项目： | 国家重大科技专项（2002AA22345B） |
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| 摘 要： | 天然二硫键的形成是许多蛋白正确折叠中的限速步骤，在稳定蛋白质构象和保持蛋白质活性方面起重要作用。讨论的二硫键异构酶是内质网中一种重要的蛋白折叠催化剂，它催化蛋白二硫键的形成和错误配对二硫键的重排，并有抑制错误折叠蛋白聚集的分子伴侣活性。PDI广泛应用于基因工程上提高外源蛋白表达水平。
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| 关 键 词： | 二硫键异构酶 蛋白折叠 蛋白聚集 分子伴侣 |
| 收稿时间： | 2003-10-31 |
| 修稿时间： | 2004-12-13 |
Protein Disulfide Isomerase |
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| XIONG Xiang-hua ,LIU Zhi-min.Protein Disulfide Isomerase[J].China Biotechnology,2005,25(B04):176-179. |
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| Authors: | XIONG Xiang-hua LIU Zhi-min |
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| Institution: | Institute of Biotechnology, Acaclemy of Military Medical Sciences Beijing 100071, China |
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| Abstract: | The formation of native disulfide bond is critical for the proper folding of many proteins. It maintains the integrity of protein structure and activity. This review focuses on one of important protein folding assistants- protein disulfide isomerase in endoplasmic reticulum. PDI, an enzyme that catalyzes disulfide formation and rearrangement of incorrect disulfide, behaves as a chaperone inhabiting the aggregation of misfolded proteins. It has a wide application in the expression of heterlogous proteins in eukaryotic hosts. |
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| Keywords: | PDI Protein folding Protein aggregation Molecular chaperone |
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