炭样小单孢菌中抗生素生物合成相关蛋白的筛选

目的：筛选一株具有广谱抗菌活性的炭样小单孢菌JXNU-1中核苷类抗生素生物合成相关蛋白。方法：通过iTRAQ定量蛋白质组学技术对JXNU-1菌体生长期（36h）和产物合成期（108h）的差异蛋白进行鉴定和功能分析。结果：基于iTRAQ定量蛋白质组学技术共鉴定出炭样小单孢菌总蛋白质2390个，差异表达蛋白172个，在产物合成期（108h）表达上调76个、表达下调96个。通过蛋白GO和COG注释等功能分析，筛选出12个与抗生素合成密切相关蛋白和5个生物合成基因簇。结论：利用iTRAQ技术筛选出炭样小单孢菌JXNU-1的抗生素合成相关蛋白，为阐明该抗生素的生物合成机制奠定实验依据。

Screening of the Protein Related to Antibiotics Biosynthesis in Micromonospora carbonacea JXNU-1

Objective:To screen the protein related to antibiotics biosynthesis in Micromonospora carbonacea JXNU-1 with broad-spectrum antimicrobial activity. Methods:iTRAQ was used to analyze the differentially expressed proteins between the time of antibiotics secretion/non-secretion in M.carbonacea JXNU-1. Results:A total of 2 390 proteins were identified using iTRAQ, and 172 proteins were differentially expressed during the antibiotics secretion time in M.carbonacea JXNU-1, including 76 up-regulated and 96 down-regulated. Twelve proteins and five gene clusters closely related to biosynthesis of antibiotics were screened based on the functional analysis of the differentially expressed proteins with GO and COG, annotation. Conclusion:The proteins related to antibiotics biosynthesis in M.carbonacea JXNU-1 have been screened based on iTRAQ, it could provide the theoretical basis for revealing the mechanism of antibiotics biosynthesis in M.carbonacea JXNU-1.