信号肽优化对重组抗体分泌表达的影响及研究进展

重组抗体药物在抗肿瘤、抗自身免疫病、抗感染等领域都有较好的应用前景,且市场的需求量正在逐年上升。然而许多的抗体药物在研究、生产过程中都面临着产量低、稳定性差、活性低等问题。目前,在大规模制备抗体药物的过程中,常常利用动物细胞表达系统来生产分泌蛋白,而信号肽作为连接在分泌蛋白N端的一段氨基酸序列,能够控制蛋白质的分泌途径进而影响抗体蛋白的表达产量。简要概述了信号肽的一级结构与作用机制,同时,指出其对重组抗体蛋白分泌效率的影响途径,最后结合各种研究成果总结出信号肽序列优化策略。

Optimized Signal Peptides Sequences for the Development of High Expressing McAbs

Recombinant antibodies have good prospects in anti-tumor, anti-autoimmune disease and other fields, the market demand is increasing year by year. However, the study of recombinant antibody are facing with many problems, such as the low yields and poor stability. The recombinant antibody was often expressed in CHO cell as a secret protein, for which the signal peptide is very important. The signal peptide is an amino acid sequence that attach to the N-terminus of immature protein, it can affect the expression of protein by controlling the secretory pathway. The structure, mechanism of the signal peptide andits functions on the recombinant protein secretion pathway were briefly outlined. In addition, the optimizing strategy of signal peptide was summarized based on a variety of researches.