草甘膦诱导表达的大豆与棉花EST序列的分离

利用差异显示PCR方法分离了63个草甘膦诱导后在大豆和棉花中差异表达的片段,测序分析结果表明属于33个草甘膦诱导的大豆和棉花EST序列。通过在GenBank中进一步比时研究发现:约85％的EST序列与水杨酸、冷、创伤、氧化等非生物胁迫诱导后表达库中的EST序列有高达95％以上的同源性,由此可推测这些基因参与了植物对非生物胁迫的反应过程。草甘膦诱导后高表达EST、序列的获得将有利于进一步分离相关非生物胁迫诱导表达基因及启动子,研究其转录调控的机理,有望建立草甘膦诱导系统。从而解决组成型表达造成外源基因在植物体所有发育阶段和所有组织部位表达,造成植物体能量浪费。

Isolation of Glyphosate-induced EST Sequences of Soybean and Cotton

63 ESTs sequences were isolated by differential display PCR from soybean and cotton seedlings. Web based alignment showed that these ESTs sequences belongs to 33 genes, and they are all about 95% homologous to abiotic factor induced genes. The results indicated that these genes involved in abiotic response process. Isolation of glyphosate-induced higher expression EST sequence will be benefit to isolate the gene and promoter, investigate the mechanism of regulation, and construct a glyphosate-inducible system. Use this system, the gene expression can be drived only when needed, and reduce the plant energy waste.