肝素酶研究进展

微生物肝素酶是一类作用于肝素和类肝素的多糖裂解酶，在低分子肝素的制备以及肝素类分子的结构解析等领域具有十分重要的应用前景。本文将结合微生物基因组测序的最新进展，综述微生物肝素酶的来源，并对肝素酶的作用机理加以讨论；结合本实验室研究对肝素酶的重组表达及其在低分子肝素生产的应用最新进展作以综述，并对肝素酶其它潜在的应用作以讨论。

Progress in the Study of Heparinases

Heparinases,a kind of polysaccharide lyases,can degrade heparin and heparin sulfate to low molecular weight polysaccharides.It has been noted that many bacteria have heparinases although only few of them have been purified and characterized.Heparinases I,II and III from Flavobacterium heparinum have been extensively studied for many years and been commercialized recently.Heparinases have some important applications in the industry and clinic as well as in the determination of heparin structure,which is a very important anticoagulant drug used world-widely.The recent progresses in isolation of heparinase-producing bacteria,genome mapping of heparinase homologs in sequenced bacteria and archaea genomes,purification of heparinases and the study of their biochemistry and regulation were reviewed.The recombinant expression of these enzymes as well as important applications of heparinases and their potential applications in the future will also be highlighted.