| 大孔吸附树脂分离纯化培养基残基中虫草素 |
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| 引用本文： | 李从镇,毛宁.大孔吸附树脂分离纯化培养基残基中虫草素[J].中国生物工程杂志,2014,34(1):90-94. |
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| 作者姓名： | 李从镇 毛宁 |
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| 作者单位： | 福建师范大学生命科学学院 工业微生物发酵技术国家地方联合工程研究中心 福州 350108 |
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| 摘 要： | 采用大孔吸附树脂分离纯化人工蛹虫草培养基残基中的虫草素,以HPLC法测定样品中虫草素含量,筛选出了适宜的大孔树脂NKA-II。研究了pH、洗脱剂乙醇体积分数等因素对该树脂吸附性能及解析性能的影响。结果表明,NKA-II型树脂纯化虫草素的最佳吸附条件为pH9,吸附流速2BV/h,该树脂对虫草素的吸附量可达到16.5mg/g。洗脱工艺条件为2.5倍树脂柱体积的50%乙醇,NKA-II大孔树脂对虫草素的解析率可达到95%以上。
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| 关 键 词： | 虫草素 大孔树脂 分离纯化 |
| 收稿时间： | 2013-11-12 |
| 修稿时间： | 2013-12-02 |
Purification of Cordycepin from Medium Residues by Macroporous Resin |
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| LI Cong-zhen,MAO Ning.Purification of Cordycepin from Medium Residues by Macroporous Resin[J].China Biotechnology,2014,34(1):90-94. |
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| Authors: | LI Cong-zhen MAO Ning |
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| Institution: | Fujian Normal University, Industrial Microbial Fermentation Technology and Area Joint National Engineering Research Center, Fuzhou 350108, China |
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| Abstract: | Seperating cordycepin from cordycep manual medium residues by macroporous resin, Filter out a suitable macroporous resin NKA-Ⅱ with HPLC. pH, eluent concentration of ethanol and other factors were researched to the influence of absorbance and desorption of the resin. When the pH value is 9, Adsorption velocity is 2BV/h, the resin has better absorption capacity which reach 16.5mg/g. When the eluent concentration of ethanol is 50% and the volume is 2.5 times of the resin bed, the resin has better desorption capacity which can reach 95%. |
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| Keywords: | Cordycepin Macroporous resin Purification |
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