产藏红花素栀子愈伤组织的诱导和筛选

为解决藏红花资源问题，研究了栀子愈伤组织的诱导，并首次应用目视法和HPLC 方法对产藏红花素细胞系进行了筛选。结果表明栀子叶子愈伤组织优化的诱导条件为: 在添加1mg/L 2,4-D和 0.25 mg/L 6-BA的MS 培养基上， 25±1℃和31.74 mmol/m2s光照强度下每天光照16小时，培养15d后可获得100%的诱导率。种子愈伤组织也易诱导，在添加0.5mg/L 2,4-D 和 0.25 mg/L 6-BA的MS培养基上，25±1℃全时暗培养15d后可获得100%的诱导率。首次应用建立的目视法和HPLC 方法, 从90株细胞系中筛选出细胞系seed4，其藏红花素1 的含量是0.348mg/g，生长较快、不易褐化，兼顾了此次生代谢产物的含量和细胞的生长速度，为采用植物细胞工程法解决藏红花素资源短缺问题提供了种质资源。

Induction and screening of Gardendia jsminoide callus lines for crocin production

Induction and screening of Gardenia jasminoides callus for croin production were investigated. When the leaves were cultured on MS medium supplemented with 1mg/L 2,4-D and 0.25 mg/L 6-BA at 25℃ under 16 hours light with the intensity of 31.74 umol/m2s per day, the induction rate was 100% after 15 days. When the seeds were cultured on MS medium supplemented with 0.5mg/L 2,4-D and 0.25 mg/L in dark for 15day, the induction rate was 100%. By eyesight and HPLC methods, one callus line (seed4) which contained 0.348mg /g crocin and would not brown was selected from 90 lines.