微生物谷氨酰胺转胺酶对大鼠创伤愈合作用的实验研究

本文研究了微生物谷氨酰胺转胺酶对大鼠创伤的促愈合作用。建立大鼠背部刀割伤模型，用创面照像、透明膜描记扫描记录伤后第5、10、15、20 天创面面积，计算创伤愈合率；并用注水法测量伤腔容积，同时观测肉芽组织再生及其总蛋白、氨基已糖和己糖醛酸的含量变化情况。结果实验组创面愈合时间平均为18.1天，较对照组平均缩短了2-3天(P<0.05)；创伤愈合率显著提高(P<0.05或P<0.01)；伤腔容积明显缩小(P<0.05)；实验组肉芽干湿重较对照组显著增加(P<0.05)，肉芽中蛋白质、氨基已糖和己糖醛酸含量增加显著(P<0.05)。结果显示谷氨酰胺转胺酶具有促进大鼠皮肤创伤愈合的作用，其作用机理可能是促进肉芽组织中蛋白质，氨基多糖和胶原的合成有关。

Efficacy of Microbial Transglutaminase on Wound Healing in Rats

Effect of microbial transglutaminase from Streptoverticillium SG215 on cutaneous wound healing in rats was evaluated using excision wound model.The wounds were visually observed,photographically documented and the wound area was measured at 5,10,15,20th days.The volume of wound was measured by water injection method,the contents of protein,hydroxyproline(Hyp),hexuranic,hexosamines in the granulation tissues and regeneration of granulation tissues were investigated.The closure time and volume of the skin wounds in the experimental groups significantly decreased when compared to that in the control group(P<0.05).Wound contraction,dry(or wet) weight of granulation tissues and content of protein,hydroxyproline,hexuranic,hexosamines in the wound tissues in the experimental groups were higher than that in the control group(P<0.05).These results clearly substantiate the beneficial effects of microbial transglutaminase in wound healing.