HepG2/mdr1稳定细胞系的建立及特性研究

将已构建好的含有人多药耐药（multidrug resistance, MDR）全长基因的真核表达质粒pCI-neo-mdr1，应用脂质体导入人肝癌HepG2细胞，应用G418筛选出人肝癌多药耐药细胞株HepG2/mdr1。通过对HepG2/mdr1细胞形态学的观察和生物学特性的研究，成功地建立了高效、稳定的HepG2/mdr1细胞系；为深入研究肝癌的MDR及其逆转提供了理想的细胞模型，并为探索建立肝癌MDR细胞株提供新的方法和思路，同时为研究肝癌细胞胰岛素抵抗与MDR的关系提供了模型细胞。 将已构建好的含有人多药耐药（multidrug resistance, MDR）全长基因的真核表达质粒pCI-neo-mdr1，应用脂质体导入人肝癌HepG2细胞，应用G418筛选出人肝癌多药耐药细胞株HepG2/mdr1。通过对HepG2/mdr1细胞形态学的观察和生物学特性的研究，成功地建立了高效、稳定的HepG2/mdr1细胞系；为深入研究肝癌的MDR及其逆转提供了理想的细胞模型，并为探索建立肝癌MDR细胞株提供新的方法和思路，同时为研究肝癌细胞胰岛素抵抗与MDR的关系提供了模型细胞。

Creation and Characteristics Research of HepG2/mdr1 Stable Cell Line

Eukaryotic expression plasmid pCI-neo-mdr1 which contains human multidrug resistance gene 1(mdr1), was constructed and transferred into human hepatocarcinoma HepG2 cells by use of liposome. G418 was used to screen the cells successfully with mdr1 and the selected cells was named HepG2/mdr1 Morphological and biological properties of HepG2/mdr1 cells were observed. The results show that the constructed HepG2/mdr1 cell line was high efficient and stationary in the expression of mdr1. The work was valuable and desirable for the establishment of multidrug resistant cell models, and for the study of MDR in human hepatoma. Furthermore, the work also provided a perfect model for the research of relationship between insulin resistance and MDR in hepatocarcinoma cells.To construct the eukaryotic expression plasmid pCI-neo-mdr1 containing multidrug resistance gene 1(mdr1), and transfer into human hepatocarcinoma cell line HepG2 cell by use the liposome, screening human hepatoma multidrug resistance (MDR) cell line HepG2/mdr1 with G418， and the biological property of HepG2/mdr1 cells was observed. The results showed that the HepG2/mdr1 cell line that we constructed is highly efficient and stationary in performance and the goal gene expression. This work was valuable and desirable for the study of MDR phenomenon and reversing measure of MDR, and exploring establish of MDR hepatocarcinoma cell line. Furthermore, The work also provided a perfect model cell for the research of relationship between insulin resistance and MDR in hepatocarcinoma cells.